Gunpowder RIVERKEEPER® Partners with Blue Water Baltimore for Little Falls Bacteria, Optical Brighteners, and Road Salt Monitoring

Wednesday December 17th, Blue Water Baltimore’s Sarah Holter and Dri DeLuca made the trip up from the city to Sparks Bank Nature Center for a day of collaboration and water quality monitoring on the Little Falls. They brought with them their YSI, Aquafluor, and a wealth of knowledge from their monitoring programs that Gunpowder RIVERKEEPER® was eager to learn from as we gear up to apply for our Chesapeake Monitoring Cooperative Tier III accreditation for nontidal and tidal sites in 2026.

The whole crew posing for a selfie as we wrapped up our monitoring session at Stabler’s Church

After a brief tour of the Nature Center and a doughnut, Brady Bayne, Tristan McGregor, and Joe Ottomano set off to the first of seven monitoring locations selected and mapped by Brady the day prior.

Map of the seven sampling sites

Armed with our sampling bottles and equipment, we arrived at a pull-off on Blue Mount Road to find the lot still covered in snow from last weekend’s storm. Undeterred, we pressed on to gather our samples and to take readings with the YSI and Aquafluor machines. We measured water temperature, barometric pressure, dissolved Oxygen, Conductivity, pH, and turbidity with the YSI probe all of which are indicators of water quality.

Sarah deploying the YSI while Tristan waded in downstream to collect samples

With the Aquafluor, we filled a cuvette with a small sample of water to determine the concentration of optical brighteners. These common additives in laundry detergents and other products are a key indicator that septic leakage is occurring in a watershed. Gunpowder RIVERKEEPER® is interested in pursuing this method to identify possible sources of contamination. We were fortunate to find amounts no larger than 3.5 parts per million, or less than 20% of the concentration that Blue Water Baltimore would report as a level of concern.

Tristan eyeballing the water level in the cuvette for the Aquafluor optical brighteners testing

Along with the quantitative measurements, we noted the quality of the banks, bank stability, benthic sediment type, and other notable features such as a large outfall beneath the Big Falls bridge, the flood plain behind the White Hall post office, and the Owl Branch tributary feeding Little Falls just above our sample site.

Sarah reading off the YSI measurements while Dri prepared the Aquafluor sample and Joe recorded on the datasheet

After collecting our data and samples at all seven sites, and giving one of the cars just a couple pushes to get out of the snow, we returned to the Nature Center to test our samples for NaCL (Sodium Chloride, aka: salt) in parts per million for the Izaak Walton League Salt Watch and for harmful bacteria in the laboratory using the IDEXX Method (Be part of the Salt Watch: request your free salt watch kit HERE).

Site LocationAir Temperature (F°)Water Temperature (C°)Optical Brightners (PPM)Barometric Pressure (mmHG)Dissolved Oxygen (%)Dissolved Oxygen
(mg/l) 
Conductivity (mS/cm)Turbidity (FNU)pH (Units)
Blue Mount  410.12.52753101.414.34262.51.57.76
Big Falls 412.43.523751.9103.414.17214.22.018.16
Post Office 410.42.435751103.614.98263.41.598.02
Graystone 410.12.457750.5104.215.2272.21.577.39
Frederick 410.42.47748.6103.319.93277.21.337.88
Dairy 410.52.967.983103.614.95259.31.277.33
Stablers 450.12.204745.1102.314.92238.62.47.88
Table disclosing YSI data collected from sampling date

Juliette Verderaime, our Youth Climate Institute member, was lucky enough to find time in her schedule to help us with the last of the samples, but once they were sealed in Quanti-Trays and set into the incubator, there was nothing left to do but wait until the next day to see how many cells fluoresced under the ultraviolet light to indicate the presence of E. coli. Results came in 18 hours later all within safe parameters for swimming, or ice-fishing, as it were.

As always, feel free to stop by the Sparks Bank Nature Center most Tuesdays-Saturdays 9-4 if you want to learn more about the work Gunpowder RIVERKEEPER® does to protect the Gunpowder Watershed and thanks to our downstream neighbors at Blue Water Baltimore for their invaluable time, experience, and all of the work their organization does to keep our waters clean.

Brady getting a high angle action shot of sampling from the Stabler’s Church bridge crossing

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